14642-1-AP

antibody from Proteintech Group

Targeting: IGF2BP3 CT98, IMP-3, IMP3

Provider product page for 14642-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [28]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 14642-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14642-1-AP, RRID:AB_2122782
Product name: IGF2BP3 antibody
Antibody type: Polyclonal
Description: KD/KO validated IGF2BP3 antibody (Cat. #14642-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IGF2BP3 protein structure - 14642-1-AP shown in red.

Submitted references Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.

Li Z, Xu Q, Huangfu N, Chen X, Zhu J
Journal of clinical laboratory analysis 2022 Jan;36(1):e24019

Stabilization of UCA1 by N6-methyladenosine RNA methylation modification promotes colorectal cancer progression.

He RZ, Jiang J, Hu X, Lei M, Li J, Luo W, Duan L, Hu Z, Mo YY, Luo DX, Peng WX
Cancer cell international 2021 Nov 22;21(1):616

Oncogenic AURKA-enhanced N(6)-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Peng F, Xu J, Cui B, Liang Q, Zeng S, He B, Zou H, Li M, Zhao H, Meng Y, Chen J, Liu B, Lv S, Chu P, An F, Wang Z, Huang J, Zhan Y, Liao Y, Lu J, Xu L, Zhang J, Sun Z, Li Z, Wang F, Lam EW, Liu Q
Cell research 2021 Mar;31(3):345-361

linc01305 promotes metastasis and proliferation of esophageal squamous cell carcinoma through interacting with IGF2BP2 and IGF2BP3 to stabilize HTR3A mRNA.

Huang GW, Chen QQ, Ma CC, Xie LH, Gu J
The international journal of biochemistry & cell biology 2021 Jul;136:106015

EGR2-mediated regulation of m⁶A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization.

Ying Y, Ma X, Fang J, Chen S, Wang W, Li J, Xie H, Wu J, Xie B, Liu B, Wang X, Zheng X, Xie L
Cell death & disease 2021 Jul 29;12(8):750

A comprehensive epigenome atlas reveals DNA methylation regulating skeletal muscle development.

Yang Y, Fan X, Yan J, Chen M, Zhu M, Tang Y, Liu S, Tang Z
Nucleic acids research 2021 Feb 22;49(3):1313-1329

LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription.

Xie X, Lin J, Fan X, Zhong Y, Chen Y, Liu K, Ren Y, Chen X, Lai D, Li X, Li Z, Tang A
Cell death & disease 2021 Feb 19;12(2):201

Clinicopathologic Characteristics of Thyroid Nodules Positive for the THADA-IGF2BP3 Fusion on Preoperative Molecular Analysis.

Morariu EM, McCoy KL, Chiosea SI, Nikitski AV, Manroa P, Nikiforova MN, Nikiforov YE
Thyroid : official journal of the American Thyroid Association 2021 Aug;31(8):1212-1218

Effects of RNA methylation N6-methyladenosine regulators on malignant progression and prognosis of melanoma.

Liu J, Zhou Z, Ma L, Li C, Lin Y, Yu T, Wei JF, Zhu L, Yao G
Cancer cell international 2021 Aug 26;21(1):453

Stabilization of oncogenic transcripts by the IGF2BP3/ELAVL1 complex promotes tumorigenicity in colorectal cancer.

Li K, Huang F, Li Y, Li D, Lin H, Ni R, Zhang Q, Zhao M, Huang S, Zou L, Huang C
American journal of cancer research 2020;10(8):2480-2494

RNA N6-methyladenosine reader IGF2BP3 regulates cell cycle and angiogenesis in colon cancer.

Yang Z, Wang T, Wu D, Min Z, Tan J, Yu B
Journal of experimental & clinical cancer research : CR 2020 Sep 29;39(1):203

Berberine inhibits proliferation and induces G0/G1 phase arrest in colorectal cancer cells by downregulating IGF2BP3.

Zhang Y, Liu X, Yu M, Xu M, Xiao Y, Ma W, Huang L, Li X, Ye X
Life sciences 2020 Nov 1;260:118413

Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.

Wang LJ, Xue Y, Li H, Huo R, Yan Z, Wang J, Xu H, Wang J, Cao Y, Zhao JZ
Journal of cellular and molecular medicine 2020 May;24(9):4981-4991

EIF4G1 and RAN as Possible Drivers for Malignant Pleural Mesothelioma.

Dell'Anno I, Barbarino M, Barone E, Giordano A, Luzzi L, Bottaro M, Migliore L, Agostini S, Melani A, Melaiu O, Catalano C, Cipollini M, Silvestri R, Corrado A, Gemignani F, Landi S
International journal of molecular sciences 2020 Jul 9;21(14)

N⁶-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.

Du J, Liao W, Liu W, Deb DK, He L, Hsu PJ, Nguyen T, Zhang L, Bissonnette M, He C, Li YC
Developmental cell 2020 Dec 21;55(6):737-753.e7

Long Noncoding RNA TINCR-Mediated Regulation of Acetyl-CoA Metabolism Promotes Nasopharyngeal Carcinoma Progression and Chemoresistance.

Zheng ZQ, Li ZX, Guan JL, Liu X, Li JY, Chen Y, Lin L, Kou J, Lv JW, Zhang LL, Zhou GQ, Liu RQ, Chen F, He XJ, Li YQ, Li F, Xu SS, Ma J, Liu N, Sun Y
Cancer research 2020 Dec 1;80(23):5174-5188

Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.

Jia C, Tang H, Yang Y, Yuan S, Han T, Fang M, Huang S, Hu R, Li C, Geng W
Biochemical and biophysical research communications 2020 Aug 13;529(1):43-50

FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression.

Hong Y, Qin H, Li Y, Zhang Y, Zhuang X, Liu L, Lu K, Li L, Deng X, Liu F, Shi S, Liu G
Journal of cellular physiology 2019 Nov;234(11):19895-19910

RNA Binding Proteins Control Transdifferentiation of Hepatic Stellate Cells into Myofibroblasts.

Wang S, Jung Y, Hyun J, Friedersdorf M, Oh SH, Kim J, Premont RT, Keene JD, Diehl AM
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(3):1215-1229

The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.

Li Z, Zhang J, Liu X, Li S, Wang Q, Di Chen, Hu Z, Yu T, Ding J, Li J, Yao M, Fan J, Huang S, Gao Q, Zhao Y, He X
Nature communications 2018 Apr 20;9(1):1572

Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase.

Zhang Y, Chen Z, Wang T, Yang J, Li R, Wang S, Liu J, Ye Z
Oncotarget 2016 Jun 28;7(26):39302-39315

SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b.

Kugel S, Sebastián C, Fitamant J, Ross KN, Saha SK, Jain E, Gladden A, Arora KS, Kato Y, Rivera MN, Ramaswamy S, Sadreyev RI, Goren A, Deshpande V, Bardeesy N, Mostoslavsky R
Cell 2016 Jun 2;165(6):1401-1415

Expression of the cancer testis antigen IGF2BP3 in colorectal cancers; IGF2BP3 holds promise as a specific immunotherapy target.

Shantha Kumara H, Kirchoff D, Caballero OL, Su T, Ahmed A, Herath SA, Njoh L, Cekic V, Simpson AJ, Cordon-Cardo C, Whelan RL
Oncoscience 2015;2(6):607-14

C1QBP negatively regulates the activation of oncoprotein YBX1 in the renal cell carcinoma as revealed by interactomics analysis.

Wang Y, Yue D, Xiao M, Qi C, Chen Y, Sun D, Zhang N, Chen R
Journal of proteome research 2015 Feb 6;14(2):804-13

Role of IGF2BP3 in trophoblast cell invasion and migration.

Li W, Liu D, Chang W, Lu X, Wang YL, Wang H, Zhu C, Lin HY, Zhang Y, Zhou J, Wang H
Cell death & disease 2014 Jan 23;5:e1025

Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models.

Nguyen LH, Robinton DA, Seligson MT, Wu L, Li L, Rakheja D, Comerford SA, Ramezani S, Sun X, Parikh MS, Yang EH, Powers JT, Shinoda G, Shah SP, Hammer RE, Daley GQ, Zhu H
Cancer cell 2014 Aug 11;26(2):248-61

Characterization and analysis of the composition and dynamics of the mammalian riboproteome.

Reschke M, Clohessy JG, Seitzer N, Goldstein DP, Breitkopf SB, Schmolze DB, Ala U, Asara JM, Beck AH, Pandolfi PP
Cell reports 2013 Sep 26;4(6):1276-87

The distribution of IGF2 and IMP3 in osteosarcoma and its relationship with angiogenesis.

Chen P, Wang SJ, Wang HB, Ren P, Wang XQ, Liu WG, Gu WL, Li DQ, Zhang TG, Zhou CJ
Journal of molecular histology 2012 Feb;43(1):63-70

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP(IGF2BP3 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using IGF2BP3 antibody 14642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas cancer using 14642-1-AP(IGF2BP3 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas cancer using 14642-1-AP(IGF2BP3 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue