PA5-96506

antibody from Invitrogen Antibodies

Targeting: IGF2BP3 CT98, IMP-3, IMP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96506

Provider

Invitrogen Antibodies

Product name

IGF2BP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KKIEQDTDTK ITISPLQELT LYNPERTITV KGNVETCAKA EEEIMKKIRE SYENDIASMN LQAHLIPGLN LNALGLFPPT SGMPPPTSGP PSAMTPPYPQ FEQSETETVH LFIPALSVGA IIGKQGQHIK QLSRFAGASI KIAPAEAPDA KVRMVIITGP PEAQFKAQGR IYGKIKEENF VSPKEEVKLE AHIRVPSFAA GRVIGKGGKT VNELQNLSSA EVVVPRDQTP DENDQVVVKI TGHFYACQVA QRKIQEILTQ VKQHQQQKAL QSGPPQSRRK; Positive Samples: HepG2, A-549, 293T, HeLa, Mouse testis; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.12 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IGF2BP3 in extracts of various cell lines using IGF2BP3 Polyclonal Antibody (Product # PA5-96506) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of IGF2BP3 was performed in L929 cells using IGF2BP3 Polyclonal Antibody (Product # PA5-96506).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded rat testis using IGF2BP3 Polyclonal Antibody (Product # PA5-96506) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded mouse testis using IGF2BP3 Polyclonal Antibody (Product # PA5-96506) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded human liver using IGF2BP3 Polyclonal Antibody (Product # PA5-96506) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of IGF2BP3 was performed in 100 µg extracts of HepG2 cells using IGF2BP3 Polyclonal Antibody (Product # PA5-96506). Western blot was performed from the immunoprecipitate using IGF2BP3 Polyclonal Antibody.