13528-1-AP

antibody from Invitrogen Antibodies

Targeting: TDRKH TDRD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13528-1-AP

Provider

Invitrogen Antibodies

Product name

TDRKH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSTERTSWT SLSTIQKIAL GFGIPASATV AYILYRRYRE SREERLTFVG EDDIEIEMRV PQEAVKLIIG RQGANIKQLR KQTGARIDVD TEDVGDERVL LISGFPVQVC KAKAAIHQIL TENTPVSEQL SVPQRSVGRI IGRGGETIRS ICKASGAKIT CDKESEGTLL LSRLIKISGT QKEVAAAKHL ILEKVSEDEE LRKRIAHSAE TRVPRKQPIS VRREDMTEPG GAGEPALWKN TSSSMEPTAP LVTPPPKAGG DMAVVVSKEG SWEKPSDDSF QKSEAQAIPE MPMFEIPSPD FSFHADEYLE VYVSASEHPN HFWIQIVGSR SLQLDKLVNE MTQHYENSVP EDLTVHVGDI VAAPLPTNGS WYRAR (1-376 aa encoded by BC032690)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NCCIT cell were subjected to SDS PAGE followed by western blot with 13528-1-AP ( TDRKH specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13528-1-AP (TDRKH specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using TDRKH antibody 13528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 13528-1-AP (TDRKH specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using TDRKH antibody 13528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13528-1-AP (TDRKH specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 13528-1-AP (TDRKH specific antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 13528-1-AP (TDRKH specific antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13528-1-AP ( TDRKH specific antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TDRKH specific (IP:13528-1-AP, 4ug; Detection:13528-1-AP 1:7000) with mouse brain tissue lysate 4000ug.