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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [35]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF1857 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Lipocalin-2/NGAL Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Lipocalin-2/NGAL in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant rat Lipocalin-2/NGAL is observed and less than 10% cross-reactivity with recombinant human (rh) Lipocalin-2/NGAL is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P11672- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Negr1 controls adult hippocampal neurogenesis and affective behaviors.
Secretomes from metastatic breast cancer cells, enriched for a prognostically unfavorable LCN2 axis, induce anti-inflammatory MSC actions and a tumor-supportive premetastatic lung.
CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury.
Early Gene Expression Profile in Retinal Ganglion Cell Layer After Optic Nerve Crush in Mice.
The IgCAM CLMP regulates expression of Connexin43 and Connexin45 in intestinal and ureteral smooth muscle contraction in mice.
Immune-Induced Fever Is Dependent on Local But Not Generalized Prostaglandin E(2) Synthesis in the Brain.
MC4R-dependent suppression of appetite by bone-derived lipocalin 2.
Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease.
Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations.
Cyclooxygenase Isoform Exchange Blocks Brain-Mediated Inflammatory Symptoms.
Beneficial Effects of Sodium Phenylbutyrate Administration during Infection with Salmonella enterica Serovar Typhimurium.
Keap1 hypomorphism protects against ischemic and obstructive kidney disease.
Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons.
A novel role for protein tyrosine phosphatase 1B as a positive regulator of neuroinflammation.
Identification of lipocalin-2 as a PKCδ phosphorylation substrate in neutrophils.
Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis.
Estrogen receptor (ER)α-regulated lipocalin 2 expression in adipose tissue links obesity with breast cancer progression.
Human α-defensin expression is not dependent on CCAAT/enhancer binding protein-ε in a murine model.
Lipocalin 2 is a regulator of macrophage polarization and NF-κB/STAT3 pathway activation.
Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2.
α-Intercalated cells defend the urinary system from bacterial infection.
mTORC1 maintains renal tubular homeostasis and is essential in response to ischemic stress.
The bacteriostatic protein lipocalin 2 is induced in the central nervous system of mice with west Nile virus encephalitis.
The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
Lipocalin 2 regulates brown fat activation via a nonadrenergic activation mechanism.
In vivo implanted bone marrow-derived mesenchymal stem cells trigger a cascade of cellular events leading to the formation of an ectopic bone regenerative niche.
No effect of NGAL/lipocalin-2 on aggressiveness of cancer in the MMTV-PyMT/FVB/N mouse model for breast cancer.
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks.
Lipocalin 2 in the central nervous system host response to systemic lipopolysaccharide administration.
ER stress drives Lipocalin 2 upregulation in prostate cancer cells in an NF-κB-dependent manner.
Lipocalin-2 is an autocrine mediator of reactive astrocytosis.
Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons.
The adipokine lipocalin 2 is regulated by obesity and promotes insulin resistance.
Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
Noh K, Lee H, Choi TY, Joo Y, Kim SJ, Kim H, Kim JY, Jahng JW, Lee S, Choi SY, Lee SJ
Molecular psychiatry 2019 Aug;24(8):1189-1205
Molecular psychiatry 2019 Aug;24(8):1189-1205
Secretomes from metastatic breast cancer cells, enriched for a prognostically unfavorable LCN2 axis, induce anti-inflammatory MSC actions and a tumor-supportive premetastatic lung.
Meade KJ, Sanchez F, Aguayo A, Nadales N, Hamalian SG, Uhlendorf TL, Banner LR, Kelber JA
Oncotarget 2019 Apr 30;10(32):3027-3039
Oncotarget 2019 Apr 30;10(32):3027-3039
CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury.
Yakulov TA, Todkar AP, Slanchev K, Wiegel J, Bona A, Groß M, Scholz A, Hess I, Wurditsch A, Grahammer F, Huber TB, Lecaudey V, Bork T, Hochrein J, Boerries M, Leenders J, de Tullio P, Jouret F, Kramer-Zucker A, Walz G
Nature communications 2018 Sep 10;9(1):3660
Nature communications 2018 Sep 10;9(1):3660
Early Gene Expression Profile in Retinal Ganglion Cell Layer After Optic Nerve Crush in Mice.
Ueno S, Yoneshige A, Koriyama Y, Hagiyama M, Shimomura Y, Ito A
Investigative ophthalmology & visual science 2018 Jan 1;59(1):370-380
Investigative ophthalmology & visual science 2018 Jan 1;59(1):370-380
The IgCAM CLMP regulates expression of Connexin43 and Connexin45 in intestinal and ureteral smooth muscle contraction in mice.
Langhorst H, Jüttner R, Groneberg D, Mohtashamdolatshahi A, Pelz L, Purfürst B, Schmidt-Ott KM, Friebe A, Rathjen FG
Disease models & mechanisms 2018 Feb 22;11(2)
Disease models & mechanisms 2018 Feb 22;11(2)
Immune-Induced Fever Is Dependent on Local But Not Generalized Prostaglandin E(2) Synthesis in the Brain.
Eskilsson A, Matsuwaki T, Shionoya K, Mirrasekhian E, Zajdel J, Schwaninger M, Engblom D, Blomqvist A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 May 10;37(19):5035-5044
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 May 10;37(19):5035-5044
MC4R-dependent suppression of appetite by bone-derived lipocalin 2.
Mosialou I, Shikhel S, Liu JM, Maurizi A, Luo N, He Z, Huang Y, Zong H, Friedman RA, Barasch J, Lanzano P, Deng L, Leibel RL, Rubin M, Nickolas T, Chung W, Zeltser LM, Williams KW, Pessin JE, Kousteni S
Nature 2017 Mar 16;543(7645):385-390
Nature 2017 Mar 16;543(7645):385-390
Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease.
Kim Y, Park SJ, Manson SR, Molina CA, Kidd K, Thiessen-Philbrook H, Perry RJ, Liapis H, Kmoch S, Parikh CR, Bleyer AJ, Chen YM
JCI insight 2017 Dec 7;2(23)
JCI insight 2017 Dec 7;2(23)
Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations.
Trudu M, Schaeffer C, Riba M, Ikehata M, Brambilla P, Messa P, Martinelli-Boneschi F, Rastaldi MP, Rampoldi L
Scientific reports 2017 Aug 7;7(1):7383
Scientific reports 2017 Aug 7;7(1):7383
Cyclooxygenase Isoform Exchange Blocks Brain-Mediated Inflammatory Symptoms.
Björk Wilhelms D, Mirrasekhian E, Zajdel J, Kumar Singh A, Engblom D
PloS one 2016;11(11):e0166153
PloS one 2016;11(11):e0166153
Beneficial Effects of Sodium Phenylbutyrate Administration during Infection with Salmonella enterica Serovar Typhimurium.
Jellbauer S, Perez Lopez A, Behnsen J, Gao N, Nguyen T, Murphy C, Edwards RA, Raffatellu M
Infection and immunity 2016 Sep;84(9):2639-52
Infection and immunity 2016 Sep;84(9):2639-52
Keap1 hypomorphism protects against ischemic and obstructive kidney disease.
Tan RJ, Chartoumpekis DV, Rush BM, Zhou D, Fu H, Kensler TW, Liu Y
Scientific reports 2016 Nov 2;6:36185
Scientific reports 2016 Nov 2;6:36185
Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons.
Deriu E, Boxx GM, He X, Pan C, Benavidez SD, Cen L, Rozengurt N, Shi W, Cheng G
PLoS pathogens 2016 May;12(5):e1005572
PLoS pathogens 2016 May;12(5):e1005572
A novel role for protein tyrosine phosphatase 1B as a positive regulator of neuroinflammation.
Song GJ, Jung M, Kim JH, Park H, Rahman MH, Zhang S, Zhang ZY, Park DH, Kook H, Lee IK, Suk K
Journal of neuroinflammation 2016 Apr 19;13(1):86
Journal of neuroinflammation 2016 Apr 19;13(1):86
Identification of lipocalin-2 as a PKCδ phosphorylation substrate in neutrophils.
Weng YC, Wang G, Messing RO, Chou WH
Journal of biomedical science 2015 Mar 20;22:21
Journal of biomedical science 2015 Mar 20;22:21
Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis.
Rucci N, Capulli M, Piperni SG, Cappariello A, Lau P, Frings-Meuthen P, Heer M, Teti A
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2015 Feb;30(2):357-68
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2015 Feb;30(2):357-68
Estrogen receptor (ER)α-regulated lipocalin 2 expression in adipose tissue links obesity with breast cancer progression.
Drew BG, Hamidi H, Zhou Z, Villanueva CJ, Krum SA, Calkin AC, Parks BW, Ribas V, Kalajian NY, Phun J, Daraei P, Christofk HR, Hewitt SC, Korach KS, Tontonoz P, Lusis AJ, Slamon DJ, Hurvitz SA, Hevener AL
The Journal of biological chemistry 2015 Feb 27;290(9):5566-81
The Journal of biological chemistry 2015 Feb 27;290(9):5566-81
Human α-defensin expression is not dependent on CCAAT/enhancer binding protein-ε in a murine model.
Glenthøj A, Dahl S, Larsen MT, Cowland JB, Borregaard N
PloS one 2014;9(3):e92471
PloS one 2014;9(3):e92471
Lipocalin 2 is a regulator of macrophage polarization and NF-κB/STAT3 pathway activation.
Guo H, Jin D, Chen X
Molecular endocrinology (Baltimore, Md.) 2014 Oct;28(10):1616-28
Molecular endocrinology (Baltimore, Md.) 2014 Oct;28(10):1616-28
Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2.
Miao Q, Ku AT, Nishino Y, Howard JM, Rao AS, Shaver TM, Garcia GE, Le DN, Karlin KL, Westbrook TF, Poli V, Nguyen H
Nature communications 2014 Jun 9;5:4088
Nature communications 2014 Jun 9;5:4088
α-Intercalated cells defend the urinary system from bacterial infection.
Paragas N, Kulkarni R, Werth M, Schmidt-Ott KM, Forster C, Deng R, Zhang Q, Singer E, Klose AD, Shen TH, Francis KP, Ray S, Vijayakumar S, Seward S, Bovino ME, Xu K, Takabe Y, Amaral FE, Mohan S, Wax R, Corbin K, Sanna-Cherchi S, Mori K, Johnson L, Nickolas T, D'Agati V, Lin CS, Qiu A, Al-Awqati Q, Ratner AJ, Barasch J
The Journal of clinical investigation 2014 Jul;124(7):2963-76
The Journal of clinical investigation 2014 Jul;124(7):2963-76
mTORC1 maintains renal tubular homeostasis and is essential in response to ischemic stress.
Grahammer F, Haenisch N, Steinhardt F, Sandner L, Roerden M, Arnold F, Cordts T, Wanner N, Reichardt W, Kerjaschki D, Ruegg MA, Hall MN, Moulin P, Busch H, Boerries M, Walz G, Artunc F, Huber TB
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 8;111(27):E2817-26
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 8;111(27):E2817-26
The bacteriostatic protein lipocalin 2 is induced in the central nervous system of mice with west Nile virus encephalitis.
Noçon AL, Ip JP, Terry R, Lim SL, Getts DR, Müller M, Hofer MJ, King NJ, Campbell IL
Journal of virology 2014 Jan;88(1):679-89
Journal of virology 2014 Jan;88(1):679-89
The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
Behnsen J, Jellbauer S, Wong CP, Edwards RA, George MD, Ouyang W, Raffatellu M
Immunity 2014 Feb 20;40(2):262-73
Immunity 2014 Feb 20;40(2):262-73
Lipocalin 2 regulates brown fat activation via a nonadrenergic activation mechanism.
Zhang Y, Guo H, Deis JA, Mashek MG, Zhao M, Ariyakumar D, Armien AG, Bernlohr DA, Mashek DG, Chen X
The Journal of biological chemistry 2014 Aug 8;289(32):22063-77
The Journal of biological chemistry 2014 Aug 8;289(32):22063-77
In vivo implanted bone marrow-derived mesenchymal stem cells trigger a cascade of cellular events leading to the formation of an ectopic bone regenerative niche.
Tasso R, Ulivi V, Reverberi D, Lo Sicco C, Descalzi F, Cancedda R
Stem cells and development 2013 Dec 15;22(24):3178-91
Stem cells and development 2013 Dec 15;22(24):3178-91
No effect of NGAL/lipocalin-2 on aggressiveness of cancer in the MMTV-PyMT/FVB/N mouse model for breast cancer.
Cramer EP, Glenthøj A, Häger M, Juncker-Jensen A, Engelholm LH, Santoni-Rugiu E, Lund LR, Laerum OD, Cowland JB, Borregaard N
PloS one 2012;7(6):e39646
PloS one 2012;7(6):e39646
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
Biomarker insights 2012;7:87-104
Biomarker insights 2012;7:87-104
Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks.
Malhotra D, Fletcher AL, Astarita J, Lukacs-Kornek V, Tayalia P, Gonzalez SF, Elpek KG, Chang SK, Knoblich K, Hemler ME, Brenner MB, Carroll MC, Mooney DJ, Turley SJ, Immunological Genome Project Consortium
Nature immunology 2012 Apr 1;13(5):499-510
Nature immunology 2012 Apr 1;13(5):499-510
Lipocalin 2 in the central nervous system host response to systemic lipopolysaccharide administration.
Ip JP, Noçon AL, Hofer MJ, Lim SL, Müller M, Campbell IL
Journal of neuroinflammation 2011 Sep 26;8:124
Journal of neuroinflammation 2011 Sep 26;8:124
ER stress drives Lipocalin 2 upregulation in prostate cancer cells in an NF-κB-dependent manner.
Mahadevan NR, Rodvold J, Almanza G, Pérez AF, Wheeler MC, Zanetti M
BMC cancer 2011 Jun 7;11:229
BMC cancer 2011 Jun 7;11:229
Lipocalin-2 is an autocrine mediator of reactive astrocytosis.
Lee S, Park JY, Lee WH, Kim H, Park HC, Mori K, Suk K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jan 7;29(1):234-49
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jan 7;29(1):234-49
Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons.
Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K
Kidney international 2009 Feb;75(3):285-94
Kidney international 2009 Feb;75(3):285-94
The adipokine lipocalin 2 is regulated by obesity and promotes insulin resistance.
Yan QW, Yang Q, Mody N, Graham TE, Hsu CH, Xu Z, Houstis NE, Kahn BB, Rosen ED
Diabetes 2007 Oct;56(10):2533-40
Diabetes 2007 Oct;56(10):2533-40
Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
André E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
The European respiratory journal 2006 Aug;28(2):275-85
The European respiratory journal 2006 Aug;28(2):275-85
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Lipocalin-2/NGAL by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin-2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Lipocalin-2/NGAL by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Lipocalin-2/NGAL at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lipocalin-2/NGAL in Mouse Mammary Gland. Lipocalin-2/NGAL was detected in perfusion fixed frozen sections of mouse mammary gland using Goat Anti-Mouse Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular areas in ducts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
