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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX632041 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX632041, RRID:AB_2784546
- Product name
- C9orf72 antibody [GT779]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- C9orf72 antibody detects C9orf72 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody (GTX632041) diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C9orf72 protein stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:250.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 £gg of C9orf72 antibody [GT779] (GTX632041).Western blot analysis was performed using C9orf72 antibody [GT779] (GTX632041).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
