66140-1-IG

antibody from Invitrogen Antibodies

Targeting: C9orf72 DENND9, DENNL72, MGC23980

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66140-1-IG

Provider

Invitrogen Antibodies

Product name

C9orf72 Monoclonal Antibody (3D2H6)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MSTLCPPPS PAVAKTEIAL SGKSPLLAAT FAYWDNILGP RVRHIWAPKT EQVLLSDGEI TFLANHTLNG EILRNAESGA IDVKFFVLSE KGVIIVSLIF DGNWNGDRST YGLSIILPQT ELSFYLPLHR VCVDRLTHII RKGRIWMHKE RQENVQKIIL EGTERMEDQG (1-222 aa encoded by BC020851)

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3D2H6

Vial size

150 µL

Concentration

1.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 66140-1-IG (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C6 cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SH-SY5Y cells using 66140-1-IG (C9orf72 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 66140-1-Ig (C9orf72 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 66140-1-Ig (C9orf72 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 66140-1-IG (C9orf72 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 66140-1-IG (C9orf72 antibody) at dilution of 1:1000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 66140-1-IG (C9orf72 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-C9orf72 (IP:66140-1-IG, 4ug; Detection:66140-1-IG 1:1000) with C6 cells lysate 1320ug.