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- References [24]
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- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 22637-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#22637-1-AP, RRID:AB_10953528
- Product name
- C9orf72 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated C9orf72 antibody (Cat. #22637-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Ablation of C9orf72 together with excitotoxicity induces ALS in rats.
C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.
Staufen1 in Human Neurodegeneration.
Expression of C9orf72 hexanucleotide repeat expansion leads to formation of RNA foci and dipeptide repeat proteins but does not influence autophagy or proteasomal function in neuronal cells.
BV-2 Microglial Cells Overexpressing C9orf72 Hexanucleotide Repeat Expansion Produce DPR Proteins and Show Normal Functionality but No RNA Foci.
G4C2 Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.
Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72.
C9orf72-associated SMCR8 protein binds in the ubiquitin pathway and with proteins linked with neurological disease.
Analysis of ALS-related proteins during herpes simplex virus-2 latent infection.
C9orf72/ALFA-1 controls TFEB/HLH-30-dependent metabolism through dynamic regulation of Rag GTPases.
Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.
C9orf72 Proteins Regulate Autophagy and Undergo Autophagosomal or Proteasomal Degradation in a Cell Type-Dependent Manner.
C9ORF72-related cellular pathology in skeletal myocytes derived from ALS-patient induced pluripotent stem cells.
A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.
Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.
Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.
Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.
Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.
C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.
The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.
C9orf72 is required for proper macrophage and microglial function in mice.
Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.
Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.
Dong W, Ma Y, Guan F, Zhang X, Chen W, Zhang L, Zhang L
The FEBS journal 2021 Mar;288(5):1712-1723
The FEBS journal 2021 Mar;288(5):1712-1723
C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.
Wang T, Liu H, Itoh K, Oh S, Zhao L, Murata D, Sesaki H, Hartung T, Na CH, Wang J
Cell metabolism 2021 Mar 2;33(3):531-546.e9
Cell metabolism 2021 Mar 2;33(3):531-546.e9
Staufen1 in Human Neurodegeneration.
Paul S, Dansithong W, Figueroa KP, Gandelman M, Scoles DR, Pulst SM
Annals of neurology 2021 Jun;89(6):1114-1128
Annals of neurology 2021 Jun;89(6):1114-1128
Expression of C9orf72 hexanucleotide repeat expansion leads to formation of RNA foci and dipeptide repeat proteins but does not influence autophagy or proteasomal function in neuronal cells.
Leskelä S, Huber N, Hoffmann D, Rostalski H, Remes AM, Takalo M, Hiltunen M, Haapasalo A
Biochimica et biophysica acta. Molecular cell research 2021 Jun;1868(7):119021
Biochimica et biophysica acta. Molecular cell research 2021 Jun;1868(7):119021
BV-2 Microglial Cells Overexpressing C9orf72 Hexanucleotide Repeat Expansion Produce DPR Proteins and Show Normal Functionality but No RNA Foci.
Rostalski H, Hietanen T, Leskelä S, Behánová A, Abdollahzadeh A, Wittrahm R, Mäkinen P, Huber N, Hoffmann D, Solje E, Remes AM, Natunen T, Takalo M, Tohka J, Hiltunen M, Haapasalo A
Frontiers in neurology 2020;11:550140
Frontiers in neurology 2020;11:550140
G4C2 Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.
Coyne AN, Zaepfel BL, Hayes L, Fitchman B, Salzberg Y, Luo EC, Bowen K, Trost H, Aigner S, Rigo F, Yeo GW, Harel A, Svendsen CN, Sareen D, Rothstein JD
Neuron 2020 Sep 23;107(6):1124-1140.e11
Neuron 2020 Sep 23;107(6):1124-1140.e11
Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72.
Zhu Q, Jiang J, Gendron TF, McAlonis-Downes M, Jiang L, Taylor A, Diaz Garcia S, Ghosh Dastidar S, Rodriguez MJ, King P, Zhang Y, La Spada AR, Xu H, Petrucelli L, Ravits J, Da Cruz S, Lagier-Tourenne C, Cleveland DW
Nature neuroscience 2020 May;23(5):615-624
Nature neuroscience 2020 May;23(5):615-624
C9orf72-associated SMCR8 protein binds in the ubiquitin pathway and with proteins linked with neurological disease.
Goodier JL, Soares AO, Pereira GC, DeVine LR, Sanchez L, Cole RN, García-Pérez JL
Acta neuropathologica communications 2020 Jul 16;8(1):110
Acta neuropathologica communications 2020 Jul 16;8(1):110
Analysis of ALS-related proteins during herpes simplex virus-2 latent infection.
Cabrera JR, Rodríguez-Izquierdo I, Jiménez JL, Muñoz-Fernández MÁ
Journal of neuroinflammation 2020 Dec 7;17(1):371
Journal of neuroinflammation 2020 Dec 7;17(1):371
C9orf72/ALFA-1 controls TFEB/HLH-30-dependent metabolism through dynamic regulation of Rag GTPases.
Ji YJ, Ugolino J, Zhang T, Lu J, Kim D, Wang J
PLoS genetics 2020 Apr;16(4):e1008738
PLoS genetics 2020 Apr;16(4):e1008738
Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.
Laflamme C, McKeever PM, Kumar R, Schwartz J, Kolahdouzan M, Chen CX, You Z, Benaliouad F, Gileadi O, McBride HM, Durcan TM, Edwards AM, Healy LM, Robertson J, McPherson PS
eLife 2019 Oct 15;8
eLife 2019 Oct 15;8
C9orf72 Proteins Regulate Autophagy and Undergo Autophagosomal or Proteasomal Degradation in a Cell Type-Dependent Manner.
Leskelä S, Huber N, Rostalski H, Natunen T, Remes AM, Takalo M, Hiltunen M, Haapasalo A
Cells 2019 Oct 10;8(10)
Cells 2019 Oct 10;8(10)
C9ORF72-related cellular pathology in skeletal myocytes derived from ALS-patient induced pluripotent stem cells.
Lynch E, Semrad T, Belsito VS, FitzGibbons C, Reilly M, Hayakawa K, Suzuki M
Disease models & mechanisms 2019 Aug 16;12(8)
Disease models & mechanisms 2019 Aug 16;12(8)
A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.
Liu Y, Wang T, Ji YJ, Johnson K, Liu H, Johnson K, Bailey S, Suk Y, Lu YN, Liu M, Wang J
Genes & development 2018 Nov 1;32(21-22):1380-1397
Genes & development 2018 Nov 1;32(21-22):1380-1397
Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.
Shi Y, Lin S, Staats KA, Li Y, Chang WH, Hung ST, Hendricks E, Linares GR, Wang Y, Son EY, Wen X, Kisler K, Wilkinson B, Menendez L, Sugawara T, Woolwine P, Huang M, Cowan MJ, Ge B, Koutsodendris N, Sandor KP, Komberg J, Vangoor VR, Senthilkumar K, Hennes V, Seah C, Nelson AR, Cheng TY, Lee SJ, August PR, Chen JA, Wisniewski N, Hanson-Smith V, Belgard TG, Zhang A, Coba M, Grunseich C, Ward ME, van den Berg LH, Pasterkamp RJ, Trotti D, Zlokovic BV, Ichida JK
Nature medicine 2018 Mar;24(3):313-325
Nature medicine 2018 Mar;24(3):313-325
Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.
Saberi S, Stauffer JE, Jiang J, Garcia SD, Taylor AE, Schulte D, Ohkubo T, Schloffman CL, Maldonado M, Baughn M, Rodriguez MJ, Pizzo D, Cleveland D, Ravits J
Acta neuropathologica 2018 Mar;135(3):459-474
Acta neuropathologica 2018 Mar;135(3):459-474
Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.
Davidson YS, Robinson AC, Rollinson S, Pickering-Brown S, Xiao S, Robertson J, Mann DMA
Amyotrophic lateral sclerosis & frontotemporal degeneration 2018 Feb;19(1-2):102-111
Amyotrophic lateral sclerosis & frontotemporal degeneration 2018 Feb;19(1-2):102-111
Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.
Frick P, Sellier C, Mackenzie IRA, Cheng CY, Tahraoui-Bories J, Martinat C, Pasterkamp RJ, Prudlo J, Edbauer D, Oulad-Abdelghani M, Feederle R, Charlet-Berguerand N, Neumann M
Acta neuropathologica communications 2018 Aug 3;6(1):72
Acta neuropathologica communications 2018 Aug 3;6(1):72
C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.
Maharjan N, Künzli C, Buthey K, Saxena S
Molecular neurobiology 2017 May;54(4):3062-3077
Molecular neurobiology 2017 May;54(4):3062-3077
The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.
Sullivan PM, Zhou X, Robins AM, Paushter DH, Kim D, Smolka MB, Hu F
Acta neuropathologica communications 2016 May 18;4(1):51
Acta neuropathologica communications 2016 May 18;4(1):51
C9orf72 is required for proper macrophage and microglial function in mice.
O'Rourke JG, Bogdanik L, Yáñez A, Lall D, Wolf AJ, Muhammad AK, Ho R, Carmona S, Vit JP, Zarrow J, Kim KJ, Bell S, Harms MB, Miller TM, Dangler CA, Underhill DM, Goodridge HS, Lutz CM, Baloh RH
Science (New York, N.Y.) 2016 Mar 18;351(6279):1324-9
Science (New York, N.Y.) 2016 Mar 18;351(6279):1324-9
Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
Sellier C, Campanari ML, Julie Corbier C, Gaucherot A, Kolb-Cheynel I, Oulad-Abdelghani M, Ruffenach F, Page A, Ciura S, Kabashi E, Charlet-Berguerand N
The EMBO journal 2016 Jun 15;35(12):1276-97
The EMBO journal 2016 Jun 15;35(12):1276-97
Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.
Burberry A, Suzuki N, Wang JY, Moccia R, Mordes DA, Stewart MH, Suzuki-Uematsu S, Ghosh S, Singh A, Merkle FT, Koszka K, Li QZ, Zon L, Rossi DJ, Trowbridge JJ, Notarangelo LD, Eggan K
Science translational medicine 2016 Jul 13;8(347):347ra93
Science translational medicine 2016 Jul 13;8(347):347ra93
Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.
Ferguson R, Serafeimidou-Pouliou E, Subramanian V
Journal of anatomy 2016 Dec;229(6):871-891
Journal of anatomy 2016 Dec;229(6):871-891
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22637-1-AP(C9orf72 antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The C9orf72 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human C9orf72. This antibody recognizes human,mouse,rat antigen. The C9orf72 antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-C9orf72 (IP:22637-1-AP, 3ug; Detection:22637-1-AP 1:300) with SH-SY5Y cells lysate 2000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human brain using 22637-1-AP(C9orf72 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human brain using 22637-1-AP(C9orf72 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
