GTX111597

antibody from GeneTex

Targeting: PFKM PFK-1, PFKX, PPP1R122

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX111597

Provider

GeneTex

Proper citation

GeneTex Cat#GTX111597, RRID:AB_1951192

Product name

Fructose 6 Phosphate Kinase antibody [C1C3]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.

Hu CM, Tien SC, Hsieh PK, Jeng YM, Chang MC, Chang YT, Chen YJ, Chen YJ, Lee EYP, Lee WH
Cell metabolism 2019 Mar 4;

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Glucose Catabolism in Liver Tumors Induced by c-MYC Can Be Sustained by Various PKM1/PKM2 Ratios and Pyruvate Kinase Activities.

Méndez-Lucas A, Li X, Hu J, Che L, Song X, Jia J, Wang J, Xie C, Driscoll PC, Tschaharganeh DF, Calvisi DF, Yuneva M, Chen X
Cancer research 2017 Aug 15;77(16):4355-4364

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX111597 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) B: H1299 7.5% SDS PAGE GTX111597 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Fructose 6 Phosphate Kinase antibody [C1C3] (GTX111597) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Fructose 6 Phosphate Kinase antibody [C1C3] (GTX111597) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using PFK (muscle)(GTX111597) antibody at 1:200 dilution.