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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF4274 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal PLUNC Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse PLUNC in direct ELISAs and Western blots. In these formats, less than 10% cross-reactivity with recombinant human PLUNC is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references α1-Antitrypsin promotes SPLUNC1-mediated lung defense against Pseudomonas aeruginosa infection in mice.
SPLUNC1/BPIFA1 contributes to pulmonary host defense against Klebsiella pneumoniae respiratory infection.
Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
Jiang D, Persinger R, Wu Q, Gross A, Chu HW
Respiratory research 2013 Nov 9;14:122
Respiratory research 2013 Nov 9;14:122
SPLUNC1/BPIFA1 contributes to pulmonary host defense against Klebsiella pneumoniae respiratory infection.
Liu Y, Bartlett JA, Di ME, Bomberger JM, Chan YR, Gakhar L, Mallampalli RK, McCray PB Jr, Di YP
The American journal of pathology 2013 May;182(5):1519-31
The American journal of pathology 2013 May;182(5):1519-31
Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
Habibzay M, Saldana JI, Goulding J, Lloyd CM, Hussell T
Mucosal immunology 2012 Sep;5(5):524-34
Mucosal immunology 2012 Sep;5(5):524-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse PLUNC by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLUNC at approximately 30kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- PLUNC in Mouse Embryo. PLUNC was detected in immersion fixed frozen sections of mouse embryo nose (13 d.p.c.) using Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
