OAAB15725

antibody from Aviva Systems Biology

Targeting: UCHL1 PARK5, PGP9.5, Uch-L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB15725

Provider

Aviva Systems Biology

Product name

UCHL1 antibody - N - terminal region

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of UCHL1 (arrow) using rabbit polyclonal UCHL1-V31 (Cat. # OAAB15725). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCHL1 gene (Lane 2) (Origene Technologies). UCHL1 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody (N-term) (Cat.# OAAB15725) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

UCHL1 Antibody (N-term) (Cat. # OAAB15725) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram) .FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.