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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB2381 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2381, RRID:AB_10678357
- Product name
- UCHL1 monoclonal antibody, clone BH7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant UCHL1.
- Isotype
- IgG
- Antibody clone number
- BH7
- Storage
- Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.
Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr
Cell 2002 Oct 18;111(2):209-18
Cell 2002 Oct 18;111(2):209-18
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
Shaw G, Morse S, Ararat M, Graham FL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Jun;16(8):869-71
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Jun;16(8):869-71
The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.
Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J
Science (New York, N.Y.) 1989 Nov 3;246(4930):670-3
Science (New York, N.Y.) 1989 Nov 3;246(4930):670-3
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blots of UCHL1 in whole cell homogenate of bovine brain stained with UCHL1 monoclonal antibody, clone BH7 (Cat # MAB2381) (right most lane), and two other monoclonal antibodies reactive with UCHL1 (left and central lane).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 monoclonal antibody, clone BH7 (Cat # MAB2381) (green) and rabbit antibody to neurofilament NF-M (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments.
- Validation comment
- Immunofluorescence
