PAB28573

antibody from Abnova Corporation

Targeting: GART GARS-AIRS-GART, PGFT, PRGS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28573

Provider

Abnova Corporation

Product name

GART polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant GART.

Antigen sequence

IEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAA
QLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSF
ILSADFPALVVKASGLAAGKGVIVAKSKEEACKAV
QEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDG
KTV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of lane 1: RT-4, lane 2: U-251 MG, lane 3: Liver and lane 4: Tonsil using GART polyclonal antibody (Cat # PAB28573).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of lane 1: NIH-3T3 and lane 2: NBT-II cell lysates using GART polyclonal antibody (Cat # PAB28573).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of U-2 OS cell line with GART polyclonal antibody (Cat # PAB28573) shows positivity in cytoplasm and mitochondria. Fixation/Permeabilization: PFA/Triton X-103

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human duodenum with GART polyclonal antibody (Cat # PAB28573) shows strong cytoplasmic positivity in glandular cells. Retrieval method: HIER pH6

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)