11054-1-AP

antibody from Invitrogen Antibodies

Targeting: MCM3AP GANP, KIAA0572, Map80, SAC3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11054-1-AP

Provider

Invitrogen Antibodies

Product name

GANP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: IGHEFSGRF FHDRRERRLG GLASQEPGAI IELFNSVLQF LASVVSSEQL CDLSWPVTEF AEAGGSRLLP HLHWNAPEHL AWLKQAVLGF QLPQMDLPPL GAPWLPVCSM VVQYASQIPS SRQTQPVLQS QVENLLHRTY CRWKSKSPSP VHGAGPSVME IPWDDLIALC INHKLRDWTP PRLPVTSEAL SEDGQICVYF FKNDLKKYDV PLSWEQARLQ TQKELQLREG RLAIKPFHPS ANNFPIPLLH MHRNWKRSTE CAQEGRIPST EDLMRGASAE ELLAQCLSSS LLLEKEENKR FEDQLQQWLS EDSGAFTDLT SLPLYLPQTL VSLSHTIEPV MKTSVTTSPQ SDMMREQLQL SEATGTCLGE RLKHLERLIR SSREEEVASE LHLSALLDMV DI (378-778 aa encoded by BC013285)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.34 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 11054-1-AP (GANP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11054-1-AP (GANP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using MCM3AP antibody 11054-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using MCM3AP antibody 11054-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 11054-1-AP (GANP antibody) at dilution of 1:10 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GANP (IP:11054-1-AP, 4ug; Detection:11054-1-AP 1:500) with mouse brain tissue lysate 4000ug.