RQ5864

antibody from NSJ Bioreagents

Targeting: ACO1 IREB1, IREBP, IRP1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5864

Provider

NSJ Bioreagents

Product name

Aconitase 1 Antibody

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the ACO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HEK293, 2) human HepG2, 3) human U-2 OS, 4) monkey liver, 5) rat liver, 6) rat kidney, 7) mouse liver and 8) mouse kidney lysate with ACO1 antibody. Predicted molecular weight ~98 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human A431 cells with ACO1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human rectal cancer with ACO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human renal cancer with ACO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat intestine with ACO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human SiHa cells with ACO1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACO1 antibody.