Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA302024 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Antibody against ALDH1A1 (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against ALDH1A1 (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ALDH1A1
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH1A1 (arrow) using rabbit polyclonal ALDH1A1 Antibody (Center) (Cat# TA302024). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of anti-ALDH1A1 Antibody (Center) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH1A1 antibody (Center)(Cat.#TA302024), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using ALDH1A1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
