ARG55353

antibody from Arigo

Targeting: ALDH1A1 ALDH1, PUMB1, RALDH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG55353

Provider

Arigo

Product name

anti-ALDH1A1 antibody

Antibody type

Monoclonal

Antigen

Human ALDH1A1 recombinant protein.

Description

Purification with Protein G.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

152CT1.2.2

Vial size

100 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 15 µg of NCI-H460 cell line lysates stained with ARG55353 anti-ALDH1A1 antibody.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 20 µg of HepG2, K562, NCI-H460 cell line (from left to right) stained with ARG55353 anti-ALDH1A1 antibody at 1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: HepG2 cells stained with ARG55353 anti-ALDH1A1 antibody (green) at 1:100 dilution, 2 h at room temperature. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ARG55353 anti-ALDH1A1 antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells. .

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: NCI-H460 cell stained with ARG55353 anti-ALDH1A1 antibody (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: formalin fixed and paraffin embedded human colon carcinoma stained with ARG55353 anti-ALDH1A1 antibody.