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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F44049 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ALDH1A1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 302-331 from the human protein was used as the immunogen for this ALDH1A1 antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the ALDH1A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH1A1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of anti-ALDH1A1 antibody and HeLa cells. Primary Ab was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with ALDH1A1 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using ALDH1A1 antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
