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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3123 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3123, RRID:AB_1571082
- Product name
- ALDH1A1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ALDH1A1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of 3-deoxyglucosone dehydrogenase as aldehyde dehydrogenase 1A1 (retinaldehyde dehydrogenase 1).
Association of ALDH1 promoter polymorphisms with alcohol-related phenotypes in Trinidad and Tobago.
Collard F, Vertommen D, Fortpied J, Duester G, Van Schaftingen E
Biochimie 2007 Mar;89(3):369-73
Biochimie 2007 Mar;89(3):369-73
Association of ALDH1 promoter polymorphisms with alcohol-related phenotypes in Trinidad and Tobago.
Moore S, Montane-Jaime K, Shafe S, Joseph R, Crooks H, Carr LG, Ehlers CL
Journal of studies on alcohol and drugs 2007 Mar;68(2):192-6
Journal of studies on alcohol and drugs 2007 Mar;68(2):192-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH1A1 (arrow) using rabbit ALDH1A1 polyclonal antibody (Cat # PAB3123). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ALDH1A1 polyclonal antibody (Cat # PAB3123) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with ALDH1A1 polyclonal antibody (Cat # PAB3123) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using ALDH1A1 polyclonal antibody (Cat # PAB3123)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
