Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- A01392 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Retinal dehydrogenase 1 ALDH1A1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for ALDH1A1 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Compound Nanoemulsion Combined with Differentiation/Cytotoxicity Drugs for Modulating Breast Cancer Stemness.
A neutrophil-biomimic platform for eradicating metastatic breast cancer stem-like cells by redox microenvironment modulation and hypoxia-triggered differentiation therapy.
Retraction of "Label-Free Isolation of Low-Adhesion Cells with Stem Properties for Cancer Stem Cell-Specific Drug Evaluation".
Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways.
Chu Y, Su B, Luo Y, Li C, Zhang Y, Liu P, Chen H, Serda M, Jiang C, Sun T
Molecular pharmaceutics 2023 Mar 6;20(3):1591-1598
Molecular pharmaceutics 2023 Mar 6;20(3):1591-1598
A neutrophil-biomimic platform for eradicating metastatic breast cancer stem-like cells by redox microenvironment modulation and hypoxia-triggered differentiation therapy.
Chu Y, Luo Y, Su B, Li C, Guo Q, Zhang Y, Liu P, Chen H, Zhao Z, Zhou Z, Wang Y, Jiang C, Sun T
Acta pharmaceutica Sinica. B 2023 Jan;13(1):298-314
Acta pharmaceutica Sinica. B 2023 Jan;13(1):298-314
Retraction of "Label-Free Isolation of Low-Adhesion Cells with Stem Properties for Cancer Stem Cell-Specific Drug Evaluation".
Pei H, Han Z, Wang Y, Xu C, Li Y, Fan Y, Li L, Tang B
Analytical chemistry 2023 Apr 11;95(14):6191
Analytical chemistry 2023 Apr 11;95(14):6191
Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways.
Li Y, Chen F, Chen J, Chan S, He Y, Liu W, Zhang G
Cancers 2020 Jan 6;12(1)
Cancers 2020 Jan 6;12(1)
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392). ALDH1A1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH1A1 Antibody (A01392) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image