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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Immunohistochemistry [1]
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- Product number
- PB9036 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-LIF Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for LIF detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. LIF information: Molecular Weight: 22008 MW; Subcellular Localization: Secreted.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Effect of Irisin on LIF and integrin αvβ3 in rats of implantation failure.
Intrauterine administration of peripheral blood mononuclear cells (PBMCs) improves endometrial receptivity in mice with embryonic implantation dysfunction.
The effects of mifepristone on the expressions of osteopontin, interleukin-6 and leukemia inhibitory factor in the villi of early pregnant women.
LIF upregulates expression of NK-1R in NHBE cells.
Zhou L, Li C, Liu X, Zhang T
Reproductive biology and endocrinology : RB&E 2021 Feb 3;19(1):18
Reproductive biology and endocrinology : RB&E 2021 Feb 3;19(1):18
Intrauterine administration of peripheral blood mononuclear cells (PBMCs) improves endometrial receptivity in mice with embryonic implantation dysfunction.
Yu N, Yang J, Guo Y, Fang J, Yin T, Luo J, Li X, Li W, Zhao Q, Zou Y, Xu W
American journal of reproductive immunology (New York, N.Y. : 1989) 2014 Jan;71(1):24-33
American journal of reproductive immunology (New York, N.Y. : 1989) 2014 Jan;71(1):24-33
The effects of mifepristone on the expressions of osteopontin, interleukin-6 and leukemia inhibitory factor in the villi of early pregnant women.
Pei K, Yu C, Shi X, Jia M
Contraception 2010 Oct;82(4):379-84
Contraception 2010 Oct;82(4):379-84
LIF upregulates expression of NK-1R in NHBE cells.
Hu CP, Feng JT, Tang YL, Zhu JQ, Lin MJ, Yu ME
Mediators of inflammation 2006;2006(5):84829
Mediators of inflammation 2006;2006(5):84829
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of LIF using anti-LIF antibody (PB9036). LIF was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIF Antibody (PB9036) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
