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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AB-449-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal LIF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human LIF is observed. Neutralizes the biological activity of recombinant mouse LIF and will also neutralize the biological activity of recombinant human LIF at a 10-fold higher IgG concentration.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Cachexia-associated adipose loss induced by tumor-secreted leukemia inhibitory factor is counterbalanced by decreased leptin.
Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate.
Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-signaling cytokines in rodents.
Arora GK, Gupta A, Narayanan S, Guo T, Iyengar P, Infante RE
JCI insight 2018 Jul 26;3(14)
JCI insight 2018 Jul 26;3(14)
Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate.
Laresgoiti U, Nikolić MZ, Rao C, Brady JL, Richardson RV, Batchen EJ, Chapman KE, Rawlins EL
Development (Cambridge, England) 2016 Oct 15;143(20):3686-3699
Development (Cambridge, England) 2016 Oct 15;143(20):3686-3699
Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-signaling cytokines in rodents.
Kanazawa H, Ieda M, Kimura K, Arai T, Kawaguchi-Manabe H, Matsuhashi T, Endo J, Sano M, Kawakami T, Kimura T, Monkawa T, Hayashi M, Iwanami A, Okano H, Okada Y, Ishibashi-Ueda H, Ogawa S, Fukuda K
The Journal of clinical investigation 2010 Feb;120(2):408-21
The Journal of clinical investigation 2010 Feb;120(2):408-21
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- LIF in Mouse Trigeminal Ganglion. LIF was detected in perfusion fixed frozen sections of mouse trigeminal ganglion using Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to sensory neurons in trigeminal ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody. Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA). The ND50 is typically 80-480 ng/mL.
