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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-47337 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LIF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from conditioned media from A375 human melanoma cell line untreated (-) or treated (+) with 100 pM recombinant human Oncostatin M/OSM and 10 pM recombinant human TNF-a for 96 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-human LIF Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47337) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for LIF at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LIF in immersion fixed paraffin-embedded sections of human Alzheimers brain. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337) using a dilution of 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LIF in immersion fixed paraffin-embedded sections of human lung array. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of LIF in TF‚1 human erythroleukemic cell line. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337). Recombinant Human LIF stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin. Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.04‚0.08 µg/mL.
