PA5-47337

antibody from Invitrogen Antibodies

Targeting: LIF CDF, DIA, HILDA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47337

Provider

Invitrogen Antibodies

Product name

LIF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, less than 30% cross-reactivity with recombinant mouse LIF is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from conditioned media from A375 human melanoma cell line untreated (-) or treated (+) with 100 pM recombinant human Oncostatin M/OSM and 10 pM recombinant human TNF-a for 96 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-human LIF Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47337) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for LIF at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LIF in immersion fixed paraffin-embedded sections of human Alzheimers brain. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337) using a dilution of 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LIF in immersion fixed paraffin-embedded sections of human lung array. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of LIF in TF‚1 human erythroleukemic cell line. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337). Recombinant Human LIF stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin. Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.04‚0.08 µg/mL.