PA5-67872

antibody from Invitrogen Antibodies

Targeting: OXGR1 aKGR, GPR80, GPR99, P2RY15, P2Y15

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-67872

Provider

Invitrogen Antibodies

Product name

OXGR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Predicted to react with Mouse and Rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

α-Ketoglutarate Upregulates Collecting Duct (Pro)renin Receptor Expression, Tubular Angiotensin II Formation, and Na(+) Reabsorption During High Glucose Conditions.

Guerrero A, Visniauskas B, Cárdenas P, Figueroa SM, Vivanco J, Salinas-Parra N, Araos P, Nguyen QM, Kassan M, Amador CA, Prieto MC, Gonzalez AA
Frontiers in cardiovascular medicine 2021;8:644797

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OXGR1 expression in Human placenta tissue lysates using a OXGR1 Polyclonal Antibody (Product # PA5-67872).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 (A) Staining of whole kidney sections showed the presence of GLUT1, (pro)renin receptor (PRR), and OXGR1 (red staining). L indicates lumen. Nuclei are stained with 4,6-diamidino-2-phenylindole dihydrochloride (DAPI). (B) Inner medullary collecting duct (IMCD) cells were grown until reach confluence and assessed for the presence of NKCC to rule out the presence of cortical tissues cells. Western blot analysis showed the presence of aquaporin (AQP)-2 in homogenates of total kidney tissue and IMCD cells. (C) NKCC was absent in IMCD. Functional IMCD cells were assessed by the activation of the vasopressin V2 with a V2 receptor agonist (DDAVP, 10 -8 M). (D) After 45 min, AQP-2 immunofluorescence was mostly detected in plasma membrane. Coimmunostaining demonstrated the coexistence of OXGR1 and PRR, which also co-localizes with AE-1, a marker of intercalated cell. (E) OXGR1 did not co-localizes with AQP-2, a marker of principal cell.