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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA800044 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800044, RRID:AB_2625171
- Product name
- TRIM9 mouse monoclonal antibody, clone OTI2D6 (formerly 2D6)
- Antibody type
- Monoclonal
- Description
- TRIM9 mouse monoclonal antibody, clone OTI2D6 (formerly 2D6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TRIM9
- Isotype
- IgG
- Antibody clone number
- OTI2D6
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references TRIM9 short isoform preferentially promotes DNA and RNA virus-induced production of type I interferon by recruiting GSK3β to TBK1.
Qin Y, Liu Q, Tian S, Xie W, Cui J, Wang RF
Cell research 2016 May;26(5):613-28
Cell research 2016 May;26(5):613-28
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (RC201423, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-TRIM9 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-TRIM9 mouse monoclonal antibody (TA800044) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM9(RC201423).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201423 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM9 antibody(TA800044), and then analyzed by flow cytometry.
- Validation comment
- FC
