PA5-23298

antibody from Invitrogen Antibodies

Targeting: RAP2A K-REV, RAP2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-23298

Provider

Invitrogen Antibodies

Product name

RAP2A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Canine, Chicken/Avian, Xenopus

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Data in support of Rap2a GTPase expression, activation and effects in LPS-mediated innate immune response and NF-κB activation.

Carvalho BC, Oliveira LC, Rocha CD, Fernandes HB, Oliveira IM, Leãõ FB, Valverde TM, Rego IMG, Ghosh S, Silva AM
Data in brief 2019 Jun;24:103965

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAP2A in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using a RAP2A polyclonal antibody (Product # PA5-23298) at 0.1 µg/mL. Goat anti-rabbit Ig HRP secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAP2A in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in RAP2A polyclonal antibody (Product # PA5-23298) using a dilution of 0.1 µg/mL followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Expression of GST-RBD-RalGDS and validation of affinity precipitation of Rap2a in mammalian cell extracts. ( a ) The expression of GST-RBD-RalGDS was obtained upon induction with 1 mM isopropyl-1-thio-beta- d -galactopyranoside (IPTG) for 2 hours of bacterial cell cultures transformed with pGEX-6P-1-RBD-RalGDS. Bacterial extracts were fractionated onto 10% SDS-PAGE, and followed by coomassie blue staining. ( b ) Coupling of GST-RBD-RalGDS to glutathione sepharose 4B (GS4B) beads. Suspensions of IPTG-induced bacterial cell cultures transformed with pGEX-6P-1 or pGEX-6P-1-RBD-RalGDS were centrifuged, lysed and sonicated. GST or GST-RBD-RalGDS fusion protein were mixed with GS4B. Bacterial cell lysates (lanes 1 and 2) and eluted samples (lanes 3-5) were separated by 10% SDS-PAGE, followed by coomassie blue staining. Two independent bacterial clones of pGEX-6P-1-RBD-RalGDS were used in lanes 4 and 5, respectively. ( c ) RAW264 cells were treated with PMA (100nM) as indicated. The lysates (500 mug) were then incubated with 100mul (~0.5mg) of bacterial lysates containing GST-RalGDS-RBD precoupled to GS4B beads. After washes, the beads mixtures were fractionated on 12% SDS-PAGE, transferred to PVDF membrane, and probed with anti-Rap2A antibody. Anti-Rabbit IgG (H + L), peroxidase conjugated was used as the secondary antibody. The detection was performed with Clarity Western ECL Blotting Substrate (BioRad) and followed by exposure to X-ray film. Densitometrical analysis of the