PAB13090

antibody from Abnova Corporation

Targeting: DIABLO DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB13090

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB13090, RRID:AB_10549298

Product name

Diablo polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of Diablo.

Antigen sequence

SDEGADQEEEAYLRED


Storage

Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway.

Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang Z, Alnemri ES
The Journal of biological chemistry 2000 Nov 17;275(46):36152-7

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Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition.

Du C, Fang M, Li Y, Li L, Wang X
Cell 2000 Jul 7;102(1):33-42

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Structural and biochemical basis of apoptotic activation by Smac/DIABLO.

Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y
Nature 2000 Aug 24;406(6798):855-62

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Diablo in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate (C) with Diablo polyclonal antibody (Cat # PAB13090) at 1 ug /mL .

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of mouse spleen tissue using Diablo polyclonal antibody (Cat # PAB13090) at 2 ug/mL .

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)