AP22217SU-S
antibody from Acris Antibodies GmbH
Targeting: DIABLO
DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP22217SU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP22217SU-S, RRID:AB_10756379
- Product name
- anti DIABLO / SMAC (full length)
- Antibody type
- Polyclonal
- Antigen
- Full-length recombinant human SMAC
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Smac/DIABLO human tissues and cell lines using AP22217SU-S at 1:2000. Legend. SMAC: full-length recombinant smac. RS1846 and Jurkat are tumor cell lines. All tissues are from normal donors except colon carcinoma (Colon Ca). SMAC expression was detected in all samples except spleen and normal colon.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human colon stained for Smac/DIABLO using at 1:2000. A, normal colon. B and C, colon carcinoma. Only trace Smac/DIABLO immunoreactivity is seen in normal colonic epithelium (A), which is seen in the apical, foveolar cells. In contrast, strong immunoreactivity is observed in nuclear (B) and cytoplasmic (C) locations in the colon carcinoma specimen. Hematoxylin-eosin counterstain.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded tissue section of human plasmacytoma (a mature B cell lymphoma) stained for Smac/DIABLO using AP22217SU-S at 1:2000. Intense nuclear staining is observed. Hematoxylin-eosin counterstain.