PA5-20221

antibody from Invitrogen Antibodies

Targeting: IL32 NK4, TAIF, TAIFb, TAIFd

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20221

Provider

Invitrogen Antibodies

Product name

IL-32 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is human spleen tissue lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human spleen lysate using a IL-32 polyclonal antibody (Product # PA5-20221) at (A) 5 and (B) 10 µg/mL. Blot shows two isoforms of IL-32.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human Spleen Lysate, showing two isoforms of IL-32. Loading: 15 µg of lysates per lane. Antibodies: IL-32 Polyclonal Antibody (Product # PA5-20221) (Lane 1: 2 µg/mL and Lane 2: 4 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-32 recombinant protein per lane. Antibodies: IL-32 Polyclonal Antibody (Product # PA5-20221) (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL, Lane 3: 2 µg/mL, and Lane 4: 4 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Observed at around 17 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of human spleen cells using a IL-32 polyclonal antibody (Product # PA5-20221) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of human spleen cells using a IL-32 polyclonal antibody (Product # PA5-20221) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with IL-32 Polyclonal Antibody (Product # PA5-20221) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with IL-32 Polyclonal Antibody (Product # PA5-20221) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human spleen tissue using IL-32 Polyclonal Antibody (Product # PA5-20221) at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-32 Polyclonal Antibody (Product # PA5-20221) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.