18121-1-AP

antibody from Invitrogen Antibodies

Targeting: FARSA CML33, FARSL, FARSLA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18121-1-AP

Provider

Invitrogen Antibodies

Product name

FARSA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MADGQVAEL LLRRLEASDG GLDSAELAAE LGMEHQAVVG AVKSLQALGE VIEAELRSTK HWELTAEGEE IAREGSHEAR VFRSIPPEGL AQSELMRLPS GKVGFSKAMS NKWIRVDKSA ADGPRVFRVV DSMEDEVQRR LQLVRGGQAE KLGEKERSEL RKRKLLAEVT LKTYWVSKGS AFSTSISKQE TELSPEMISS GSWRDRPFKP YNFLAHGVLP DSGHLHPLLK VRSQFRQIFL EMGFTEMPTD NFIESSFWNF DALFQPQQHP ARDQHDTFFL RDPAEALQLP MDYVQRVKRT H (1-300 aa encoded by BC006495)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.4 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using FARSA antibody 18121-1-AP at 1:25 dilution and FITC- labeled Donkey anti-Rabbit IgG (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using FARSA antibody 18121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using FARSA antibody 18121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-FARSA (IP:18121-1-AP, 4ug; Detection:18121-1-AP 1:2000) with MCF-7 cells lysate 3240 ug.