UM800068

antibody from Invitrogen Antibodies

Targeting: RTRAF C14orf166, CGI-99, CLE, CLE7, hCLE1, LCRP369, RLLM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

UM800068

Provider

Invitrogen Antibodies

Product name

RTRAF Monoclonal Antibody (UMAB175), UltraMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

UMAB175

Vial size

100 µL

Concentration

0.5-1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of cell lysates (35 µg) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: Jurkat, 6: MDCK, 7: COS7, 8: PC-12, 9: MCF7).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of cell lysates (35 µg) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: Jurkat, 6: MDCK, 7: COS7, 8: PC-12, 9: MCF7).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-RTRAF mouse monoclonal antibody (UM800068, green, 1:100). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human prostate tissue using anti-RTRAF mouse monoclonal antibody. (UM800068; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human tonsil using anti-RTRAF mouse monoclonal antibody. (UM800068; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human skin tissue using anti-RTRAF mouse monoclonal antibody. (UM800068; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human embryonic cerebellum using anti-RTRAF mouse monoclonal antibody. (UM800068; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-RTRAF mouse monoclonal antibody (UM800068). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.