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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA503723 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503723, RRID:AB_11126641
- Product name
- SEC14L2 ( SEC14-like 2) mouse monoclonal antibody, clone OTI4H2 (formerly 4H2)
- Antibody type
- Monoclonal
- Description
- SEC14L2 ( SEC14-like 2) mouse monoclonal antibody, clone OTI4H2 (formerly 4H2)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SEC14L2
- Isotype
- IgG
- Antibody clone number
- OTI4H2
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references SEC14L2 enables pan-genotype HCV replication in cell culture.
Saeed M, Andreo U, Chung HY, Espiritu C, Branch AD, Silva JM, Rice CM
Nature 2015 Aug 27;524(7566):471-5
Nature 2015 Aug 27;524(7566):471-5
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SEC14L2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SEC14L2 mouse monoclonal antibody (TA503723) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2(RC215994).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503723)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503723)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC215994 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SEC14L2 antibody(TA503723), and then analyzed by flow cytometry.
- Validation comment
- FC
