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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP50377PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP50377PU-N, RRID:AB_11150440
- Product name
- anti IAP4 / BIRC5 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 52-83 amino acids from the Central region of human BIRC5
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- BIRC5 Antibody (Center) (Cat. #AP50377PU-N) western blot analysis in MCF-7 cell line lysates (35µg/lane).This demonstrates the BIRC5 antibody detected the BIRC5 protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of BIRC5 (arrow) using rabbit polyclonal BIRC5 Antibody (Center) (Cat. #AP50377PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BIRC5 gene.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- BIRC5 Antibody (Center) (Cat. #AP50377PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BIRC5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- BIRC5 Antibody (Center) (Cat. #AP50377PU-N) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
