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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 645101 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#645101, RRID:AB_2068901
- Product name
- Purified anti-Caspase-1
- Antibody type
- Monoclonal
- Antigen
- Bacterial fusion protein (p20)
- Description
- Caspase -1 plays a vital role in cleaving the inactive procytokine pro-IL-1 beta to generate the active 17 kd mature form of IL-1beta, a key mediator of inflammation. Active caspase-1 (ICE) is derived a single proenzyme of 45 Kd. An active ICE comprises two subunits of P20 and two subunits of P10 after removal a large amino-terminal pro-domain and a small linker peptide. Caspase-1 is reported to be involved in CD95- mediated apoptosis. Four alternatively spliced variants of caspase-1 have been reported, with mw ranging from 30-48 kd.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- MCF7(1) and Jurkat (2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb anti-capsase 1 (clone 5B10) antibody. Proteins were visualized using a goat anti-rat secondary antibody conjugated to HRP and a chemiluminescence system.
- Conjugate
- Unconjugated
