Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- ALX-804-530-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-530-C100, RRID:AB_2050921
- Product name
- Caspase-1 (mouse) monoclonal antibody (1H11)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 1H11
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Apoptosis and non-inflammatory phagocytosis can be induced by mitochondrial damage without caspases.
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death.
van Delft MF, Smith DP, Lahoud MH, Huang DC, Adams JM
Cell death and differentiation 2010 May;17(5):821-32
Cell death and differentiation 2010 May;17(5):821-32
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death.
Marsden VS, Kaufmann T, O'reilly LA, Adams JM, Strasser A
Blood 2006 Mar 1;107(5):1872-7
Blood 2006 Mar 1;107(5):1872-7
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Supportive validation
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- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Figure A: MAbs to caspase-1 (clone 1H11 (Prod.No. ALX-804-530) and clone 4G8 (Prod. No. ALX-804-531)) detect overexpressed caspase-1 in 293T cells transiently transfected with an EE tagged inactive cysteine mutant of mouse caspase-1 by flow cytometry. . Figure B: MAbs to caspase-1 (clone 1H11 (Prod.No. ALX-804-530) and clone 4G8 (Prod. No. ALX-804-531)) detect overexpressed caspase-1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse caspase-1 as a band of ~45 kDa. Figure C: MAbs to caspase-1 (clone 1H11 (Prod.No. ALX-804-530) and clone 4G8 (Prod. No. ALX-804-531)) detect endogenous pro-caspase-1 in spleen from WT mice, but not from spleen of caspase-1 deficient mice. HSP-70: loading control.