Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1052 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EN-RAGE/S100A12 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human EN-RAGE in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P80511
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis.
In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells.
Arumugam T, Ramachandran V, Gomez SB, Schmidt AM, Logsdon CD
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Aug 15;18(16):4356-64
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Aug 15;18(16):4356-64
In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells.
Sharma I, Dhawan V, Mahajan N, Saha SC, Dhaliwal LK
Fertility and sterility 2010 Oct;94(5):1639-46.e1
Fertility and sterility 2010 Oct;94(5):1639-46.e1
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human EN-RAGE/S100A12 by Western Blot. Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EN-RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EN-RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EN-RAGE/S100A12 in Human Tonsil. EN-RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human EN-RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.