PA5-28640

antibody from Invitrogen Antibodies

Targeting: ZNF703 FLJ14299, NLZ1, ZEPPO1, ZNF503L, Zpo1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28640

Provider

Invitrogen Antibodies

Product name

ZNF703 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, A431, ZNF703-transfected 293T.

Concentration

0.58 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ZNF703 using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ZNF703 polyclonal antibody (Product # PA5-28640) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ZNF703 using 30 µg of A) Non-transfected HeLa lysates and B) ZNF703 transfected HeLa lysates. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ZNF703 polyclonal antibody (Product # PA5-28640) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ZNF703 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZNF703 Polyclonal Antibody (Product # PA5-28640) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ZNF703 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZNF703 Polyclonal Antibody (Product # PA5-28640) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ZNF703 Polyclonal Antibody detects ZNF703 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZNF703 protein stained by ZNF703 Polyclonal Antibody (Product # PA5-28640) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ZNF703 Polyclonal Antibody detects ZNF703 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ZNF703 Polyclonal Antibody (Product # PA5-28640) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.