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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- LS-C169413 - Provider product page
- Provider
- LSBio
- Product name
- Anti-PHB / Prohibitin Antibody (clone 5H7) LS-C169413
- Antibody type
- Monoclonal
- Description
- Ascites
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5H7
- Vial size
- 100µl
- Storage
- Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot using PHB monoclonal antibody against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot using PHB mouse monoclonal antibody against A431 (1), MCF-7 (2), Jurkat (3), HeLa (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded rectum cancer tissues using PHB mouse monoclonal antibody with DAB staining.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded liver cancer tissues using PHB mouse monoclonal antibody with DAB staining.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded lung cancer tissues using PHB mouse monoclonal antibody with DAB staining.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded stomach cancer tissues using PHB mouse monoclonal antibody with DAB staining.
