Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX60495 - Provider product page
- Provider
- GeneTex
- Product name
- Prohibitin antibody [5H7]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
Submitted references Targeting prohibitins at the cell surface prevents Th17-mediated autoimmunity.
Buehler U, Schulenburg K, Yurugi H, Ć olman M, Abankwa D, Ulges A, Tenzer S, Bopp T, Thiede B, Zipp F, Rajalingam K
The EMBO journal 2018 Aug 15;37(16)
The EMBO journal 2018 Aug 15;37(16)
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Supportive validation
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- Experimental details
- WB analysis of (1) A431, (2) MCF-7, (3) Jurkat, (4) HeLa, (5) HepG2, (6) A549, (7) NIH3T3, (8) Cos7 and (9) PC-12 lysates using PHB antibody [5H7].
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- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of human PHB (a.a. 68-259) recombinant protein using PHB antibody [5H7].
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- ELISA analysis of control antigen and antigen using PHB antibody [5H7].
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of NIH3T3 cells using PHB antibody [5H7] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of liver cancer tissues using PHB antibody [5H7].
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of lung cancer tissues using PHB antibody [5H7].
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- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of rectum cancer tissues using PHB antibody [5H7].
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- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of stomach cancer tissues using PHB antibody [5H7].
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of MCF-7 cells using PHB antibody [5H7] (blue) or a negative control (red).