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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB2547 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2547, RRID:AB_1578682
- Product name
- PHB polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PHB.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Prohibitin silencing reverses stabilization of mitochondrial integrity and chemoresistance in ovarian cancer cells by increasing their sensitivity to apoptosis.
The PHB1/2 phosphocomplex is required for mitochondrial homeostasis and survival of human T cells.
Assignment of the human prohibitin gene (PHB) to chromosome 17 and identification of a DNA polymorphism.
Gregory-Bass RC, Olatinwo M, Xu W, Matthews R, Stiles JK, Thomas K, Liu D, Tsang B, Thompson WE
International journal of cancer 2008 May 1;122(9):1923-30
International journal of cancer 2008 May 1;122(9):1923-30
The PHB1/2 phosphocomplex is required for mitochondrial homeostasis and survival of human T cells.
Ross JA, Nagy ZS, Kirken RA
The Journal of biological chemistry 2008 Feb 22;283(8):4699-713
The Journal of biological chemistry 2008 Feb 22;283(8):4699-713
Assignment of the human prohibitin gene (PHB) to chromosome 17 and identification of a DNA polymorphism.
White JJ, Ledbetter DH, Eddy RL Jr, Shows TB, Stewart DA, Nuell MJ, Friedman V, Wood CM, Owens GA, McClung JK
Genomics 1991 Sep;11(1):228-30
Genomics 1991 Sep;11(1):228-30
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PHB1 (arrow) using rabbit PHB polyclonal antibody (Cat # PAB2547). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with PHB polyclonal antibody (Cat # PAB2547), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of ATDC5 cells using PHB polyclonal antibody (Cat # PAB2547)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
