603102

antibody from BioLegend

Targeting: PHB PHB1

Western blot (Data presented on provider website)

Antibody Data

Product number

603102

Provider

BioLegend

Proper citation

BioLegend Cat#603102, RRID:AB_2164630

Product name

Purified anti-Prohibitin

Antibody type

Polyclonal

Antigen

Recombinant (partial), C-terminal

Description

Prohibitin is a 30 kD member of the band 7 family containing a SPFH domain. Prohibitin is localized the nucleus and mitochondrial inner cell membrane. This protein functions as a negative regulator of cell proliferation associated with senescence and cell death and a transcriptional repressor via recruitment of Brg-1, Brm or N-CoR, or HDAC1. Prohibitin can also function as a membrane-bound chaperone for the assembly of the mitochondrial respiratory chain complex. Prohibitin is induced by metabolic stress caused by imbalance in synthesis of mitochondrial-, nuclear-encoded mitochondrial proteins. Prohibitin can be modified by phosphorylation and ubiquitination. This protein has been shown to interact with Rb, Brg-1, Brm, N-CoR, HDAC1, and PHB2. The Poly6031 antibody recognizes the C-terminal region of human prohibitin and has been shown to be useful for Western blotting.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

200 µl

Storage

Upon receipt, store frozen at -20° C.

References

Submitted references

Human C4orf14 interacts with the mitochondrial nucleoid and is involved in the biogenesis of the small mitochondrial ribosomal subunit.

He J, Cooper HM, Reyes A, Di Re M, Kazak L, Wood SR, Mao CC, Fearnley IM, Walker JE, Holt IJ
Nucleic acids research 2012 Jul 1;40(13):6097-108

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Rescue of paclitaxel sensitivity by repression of Prohibitin1 in drug-resistant cancer cells.

Patel N, Chatterjee SK, Vrbanac V, Chung I, Mu CJ, Olsen RR, Waghorne C, Zetter BR
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 9;107(6):2503-8

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Up-regulation of the peroxiredoxin-6 related metabolism of reactive oxygen species in skeletal muscle of mice lacking neuronal nitric oxide synthase.

Da Silva-Azevedo L, Jähne S, Hoffmann C, Stalder D, Heller M, Pries AR, Zakrzewicz A, Baum O
The Journal of physiology 2009 Feb 1;587(Pt 3):655-68

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Coupling of the de novo fatty acid biosynthesis and lipoylation pathways in mammalian mitochondria.

Witkowski A, Joshi AK, Smith S
The Journal of biological chemistry 2007 May 11;282(19):14178-85