Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 10675-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAIP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: EQFAETLNG CVTTDDALQE LVELIYQQAT SIPNFSYMGA RLCNYLSHHL TISPQSGNFR QLLLQRCRTE YEVKDQAAKG DEVTRKRFHA FVLFLGELYL NLEIKGTNGQ VTRADILQVG LRELLNALFS NPMDDNLICA VKLLKLTGSV LEDAWKEKGK MDMEEIIQRI ENVVLDANCS RDVKQMLLKL VELRSSNWGR VHATSTYREA TPENDPNYFM NEPTFYTSDG VPFTAADPDY QEKYQELLER EDFFPDYEEN GTDLSGAGDP YLDDIDDEMD PEIEEAYEKF CLESERKRKQ (180-479 aa encoded by BC005295)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COS-7 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using PAIP1 antibody 10675-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PAIP1 (IP:10675-1-AP, 4ug; Detection:10675-1-AP 1:300) with HeLa cells lysate 3000 ug.