HPA003881

antibody from Atlas Antibodies

Targeting: PBX1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA003881

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003881, RRID:AB_1855020

Product name

Anti-PBX1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

YSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNK
RIRYKKNIGKFQEEANIYAAKTAVTATNVSAHGSQ
ANSPSTPNSAGSSSSFNMSNSGDLFMSVQSLNGDS
YQGAQVGANVQSQVDTLRHVISQTGGYSDGLAASQ
MYSP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Copy number aberrations of genes regulating normal thymus development in thymic epithelial tumors.

Petrini I, Wang Y, Zucali PA, Lee HS, Pham T, Voeller D, Meltzer PS, Giaccone G
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Apr 15;19(8):1960-71

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400918)

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.

Sample type

HUMAN