PA5-118234

antibody from Invitrogen Antibodies

Targeting: RALY HNRPCL2, P542

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118234

Provider

Invitrogen Antibodies

Product name

RALY Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of RALY in Lane A: HeLa Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: A549 Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-118234) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 32 kDa, Observed band size: 37 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RALY Polyclonal Antibody (Product # PA5-118234) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 37 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of RALY in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with RALY Polyclonal Antibody (Product # PA5-118234, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RALY Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 µL with RALY Polyclonal Antibody (Product # PA5-118234) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: RALY Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 40 kDa.