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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 13553-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PES1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LYQLLNLHY PPKLEGQAQA EAKAGEGTYA LDSESCMEKL AALSASLARV VVPATEEEAE VDEFPTDGEM SAQEEDRRKE LEAQEKHKKL FEGLKFFLNR EVPREALAFI IRSFGGEVSW DKSLCIGATY DVTDSRITHQ IVDRPGQQTS VIGRCYVQPQ WVFDSVNARL LLPVAEYFSG VQLPPHLSPF VTEKEGDYVP PEKLKLLALQ RGEDPGNLNE SEEEEEEDDN NEGDGDEEGE NEEEEEDAEA GSEKEEEARL AALEEQRMEG KKPRVMAGTL KLEDKQRLAQ EEESEAKRLA IMMMKKREKY LYQKIMFGKR RKIREANKLA EKRKAHDEAV RSEKKAKKAR PE (238-588 aa encoded by BC032489)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:300.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 13553-1-AP (PES1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13553-1-AP ( PES1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
