PA5-118192

antibody from Invitrogen Antibodies

Targeting: PRKAR2B PRKAR2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118192

Provider

Invitrogen Antibodies

Product name

PRKAR2B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PRKAR2B Polyclonal Antibody (Product # PA5-118192) at 1:500 dilution. Lane A: NIH/3T3 Whole Cell Lysate, Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 54 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PRKAR2B Polyclonal Antibody (Product # PA5-118192) at 1:500 dilution. Lane A: NIH/3T3 Whole Cell Lysate, Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 54 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PRKAR2B in human adrenal gland with PRKAR2B Polyclonal Antibody (Product # PA5-118192, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PRKAR2B in human testis with PRKAR2B Polyclonal Antibody (Product # PA5-118192, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PRKAR2B Immunoprecipitation using: Lane A: 0.5 mg NIH/3T3 Whole Cell Lysate 4 µL with PRKAR2B Polyclonal Antibody (Product # PA5-118192) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PRKAR2B Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 54 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PRKAR2B Immunoprecipitation using: Lane A: 0.5 mg NIH/3T3 Whole Cell Lysate 4 µL with PRKAR2B Polyclonal Antibody (Product # PA5-118192) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PRKAR2B Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 54 kDa.