GTX25829

antibody from GeneTex

Targeting: PRKAR2B PRKAR2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX25829

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25829, RRID:AB_380708

Product name

PKA 2 beta antibody

Antibody type

Polyclonal

Antigen

Synthetic phosphopeptide derived from a region of human PKA regulatory 2 beta subunit that contains serine 114.

Host

Rabbit

Isotype

IgG

Vial size

50µl

Storage

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysates prepared from 3T3-L1 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, and incubated with PKA regIIbeta [pS114] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that the peptide corresponding to PKA regIIbeta [pS114] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific

Validation comment

WB