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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- BS-0689R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PEPT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse large intestine lysates probed with PEPT1 Polyclonal Antibody, unconjugated (bs-0689R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HCCLM3 cells probed with Rabbit Anti-PEPT1 Polyclonal Antibody, Unconjugated (bs-0689R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-sy5y (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PEPT1 Antibody (bs-0689R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
