NB100-74653

antibody from Novus Biologicals

Targeting: ZNF106 SH3BP3, ZFP106, ZNF474

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-74653

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-74653, RRID:AB_1050099

Product name

Rabbit Polyclonal ZFP106 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ZFP106 Antibody [NB100-74653] - Detection of Human and Mouse ZFP106 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells, and whole cell lysate (50 ug) from Jurkat, Ramos, and Mouse CT26.WT cells. Antibody: Affinity purified rabbit anti-ZFP106 antibody NB100-74653 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ZFP106 Antibody [NB100-74653] - Detection of Human ZFP106 on HeLa whole cell lysate using NB100-74653. ZFP106 was also immunoprecipitated by rabbit anti-ZFP106 antibody NB100-74654.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: ZFP106 Antibody [NB100-74653] - Detection of human ZFP106 by western blot of immunoprecipitates. Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-ZFP106 antibody NB100-74653 used for IP at 6 ug per reaction. ZFP106 was also immunoprecipitated by rabbit anti-ZFP106 antibody NB100-74654. For blotting immunoprecipitated ZFP106, NB100-74653 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.