NB110-89726

antibody from Novus Biologicals

Targeting: S100A9 60B8AG, CAGB, CFAG, CGLB, LIAG, MAC387, MIF, MRP14, NIF, P14

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB110-89726

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-89726, RRID:AB_1217846

Product name

Rabbit Polyclonal S100A9 Antibody

Antibody type

Polyclonal

Description

Unpurified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Pretreatment tumor-related leukocytosis misleads positron emission tomography-computed tomography during lymph node staging in gynecological malignancies.

Mabuchi S, Komura N, Sasano T, Shimura K, Yokoi E, Kozasa K, Kuroda H, Takahashi R, Kawano M, Matsumoto Y, Kato H, Hatazawa J, Kimura T
Nature communications 2020 Mar 13;11(1):1364

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Prediction of crucial epigenetically‑associated, differentially expressed genes by integrated bioinformatics analysis and the identification of S100A9 as a novel biomarker in psoriasis.

Wang X, Liu X, Liu N, Chen H
International journal of molecular medicine 2020 Jan;45(1):93-102

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Inhibition of keratinocyte necroptosis mediated by RIPK1/RIPK3/MLKL provides a protective effect against psoriatic inflammation.

Duan X, Liu X, Liu N, Huang Y, Jin Z, Zhang S, Ming Z, Chen H
Cell death & disease 2020 Feb 19;11(2):134

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Narciclasine improves outcome in sepsis among neonatal rats via inhibition of calprotectin and alleviating inflammatory responses.

Kingsley MK, Bhat BV, Badhe BA, Dhas BB, Parija SC
Scientific reports 2020 Feb 19;10(1):2947

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Role of S100A9 in the development of neutrophilic inflammation in asthmatics and in a murine model.

Lee TH, Chang HS, Bae DJ, Song HJ, Kim MS, Park JS, Jun JA, Lee SY, Uh ST, Kim SH, Park CS
Clinical immunology (Orlando, Fla.) 2017 Oct;183:158-166

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Molecular analysis of the mouse brain exposed to chronic mild stress: The influence of hepatocyte nuclear factor 4α on physiological homeostasis.

Ikubo K, Yamanishi K, Doe N, Hashimoto T, Sumida M, Watanabe Y, El-Darawish Y, Li W, Okamura H, Yamanishi H, Matsunaga H
Molecular medicine reports 2017 Jul;16(1):301-309

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The calcium-binding proteins S100A8 and S100A9 initiate the early inflammatory program in injured peripheral nerves.

Chernov AV, Dolkas J, Hoang K, Angert M, Srikrishna G, Vogl T, Baranovskaya S, Strongin AY, Shubayev VI
The Journal of biological chemistry 2015 May 1;290(18):11771-84

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Inflammation-induced S100A8 activates Id3 and promotes colorectal tumorigenesis.

Zhang X, Ai F, Li X, She X, Li N, Tang A, Qin Z, Ye Q, Tian L, Li G, Shen S, Ma J
International journal of cancer 2015 Dec 15;137(12):2803-14

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The host protein calprotectin modulates the Helicobacter pylori cag type IV secretion system via zinc sequestration.

Gaddy JA, Radin JN, Loh JT, Piazuelo MB, Kehl-Fie TE, Delgado AG, Ilca FT, Peek RM, Cover TL, Chazin WJ, Skaar EP, Scott Algood HM
PLoS pathogens 2014 Oct;10(10):e1004450

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Involvement of Nrf2 activation in the upregulation of S100A9 by exposure to inorganic arsenite.

Sumi D, Shimizu Y, Himeno S
International journal of molecular medicine 2013 Jan;31(1):259-64

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Tumor microenvironment-derived proteins dominate the plasma proteome response during breast cancer induction and progression.

Pitteri SJ, Kelly-Spratt KS, Gurley KE, Kennedy J, Buson TB, Chin A, Wang H, Zhang Q, Wong CH, Chodosh LA, Nelson PS, Hanash SM, Kemp CJ
Cancer research 2011 Aug 1;71(15):5090-100

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: S100A9 Antibody [NB110-89726] - Simple Western lane view shows a specific band for S100A9 in 0.5 mg/ml of Human PBMC's lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY S100A9 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A9.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: S100A9 Antibody [NB110-89726] - Analysis of S100A9 Antibody in DMSO treated HL60 whole cell lysates.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200x magnification) of S100a9, in normal control, IgG Ab, and anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 um. Staining scores were determined by semi-quantitative optical analysis. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fimmu.2017.01774/full), licensed under a CC-BY licence.