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LearnNB600-281
antibody from Novus Biologicals
Targeting: ASH2L
ASH2, ASH2L1, ASH2L2, Bre2
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NB600-281 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-281, RRID:AB_10002151
- Product name
- Rabbit Polyclonal ASH2L Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein.
Bieluszewska A, Weglewska M, Bieluszewski T, Lesniewicz K, Poreba E
The FEBS journal 2018 Mar;285(5):947-964
The FEBS journal 2018 Mar;285(5):947-964
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-281] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ASH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: ASH2L Antibody [NB600-281] - ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-281 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products abeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.
